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The effects of different concentrations of azole on assembly of OXPHOS protein complex in mitochondria of Candida albicans . Each electron transport chain complex was visualized ( a ) and quantified ( b ) by <t>BN-PAGE</t> analysis of indicate condition. ( c ) ATP levels of resistance strains and normal strains ( C. albicans , N. glabrata, and C. auris ).
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The effects of different concentrations of azole on assembly of OXPHOS protein complex in mitochondria of Candida albicans . Each electron transport chain complex was visualized ( a ) and quantified ( b ) by BN-PAGE analysis of indicate condition. ( c ) ATP levels of resistance strains and normal strains ( C. albicans , N. glabrata, and C. auris ).

Journal: Microbiology Spectrum

Article Title: Divergent mitochondrial responses and metabolic signal pathways secure the azole resistance in Crabtree-positive and negative Candida species

doi: 10.1128/spectrum.04042-23

Figure Lengend Snippet: The effects of different concentrations of azole on assembly of OXPHOS protein complex in mitochondria of Candida albicans . Each electron transport chain complex was visualized ( a ) and quantified ( b ) by BN-PAGE analysis of indicate condition. ( c ) ATP levels of resistance strains and normal strains ( C. albicans , N. glabrata, and C. auris ).

Article Snippet: Twenty microliters of each sample (60 to 80 µg of protein) was loaded onto a Blue native polyacrylamide gel electrophoresis (BN-PAGE) gradient gel (4%–16%) (Invitrogen, Inc.).

Techniques: